Western blot analysis of SPOP in extracts of various cell lines. Samples were incubated with SPOP Polyclonal antibody (Product # PA5-116544) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Western blot analysis of SPOP using SPOP Polyclonal Antibody (Product # PA5-116544) at a 1:3,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supplier Page from Thermo Fisher Scientific for SPOP Antibody